Clinical features and molecular epidemiology of CMY-type beta-lactamase-producing Escherichia coli.

نویسندگان

  • Hanna E Sidjabat
  • David L Paterson
  • Zubair A Qureshi
  • Jennifer M Adams-Haduch
  • Alexandra O'Keefe
  • Alvaro Pascual
  • Jesús Rodríguez-Baño
  • Yohei Doi
چکیده

BACKGROUND Knowledge of the clinical features of infections caused by Escherichia coli strains that produce plasmid-mediated AmpC beta-lactamase is limited. Of the several groups of plasmid-mediated AmpC beta-lactamases, CMY-type beta-lactamase is the most common in the United States. METHODS We prospectively identified patients infected or colonized with E. coli strains that produce CMY-type beta-lactamase, and we collected clinical data over a 7-month period. A retrospective cohort study was performed to identify features associated with these cases. Patients with extended-spectrum beta-lactamase-producing E. coli were used as a control group. Pulsed-field gel electrophoresis, plasmid analysis, and phylogenetic typing were performed. RESULTS Twenty-two patients with infection or colonization due to CMY-type beta-lactamase-producing E. coli and 25 patients with infection or colonization due to extended-spectrum beta-lactamase-producing E. coli were identified. The demographic characteristics of the patients were similar in both cohorts. Patients with CMY-type beta-lactamase-producing E. coli were significantly more likely to have symptomatic infection than were patients with extended-spectrum beta-lactamase-producing E. coli (P = .028). The CMY-type beta-lactamase was identified as CMY-2 or its variants. Ninety-four percent of the CMY-type beta-lactamase-producing isolates belonged to E. coli phylogenetic groups B2 and D, which are associated with virulence. Many of the isolates shared similar plasmid profiles, whereas the pulsed-field gel electrophoresis profiles were diverse. Co-resistance to non-beta-lactam antimicrobials was common. CONCLUSION In Pittsburgh, Pennsylvania, CMY-type beta-lactamase-producing E. coli strains are almost as common as extended-spectrum beta-lactamase-producing E. coli strains, and they cause symptomatic infection in the majority of cases.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Prevalence and Molecular Characterization of Plasmid-mediated Extended-Spectrum β-Lactamase Genes (balaTEM, blaCTX and blASHV) Among Urinary Escherichia coli Clinical Isolates in Mashhad, Iran

Objective(s) Extended-spectrum beta-lactamase (ESBL) producing bacteria have an important role in nosocomial infections. Due to the limited availability of information about the molecular epidemiology of ESBL producing bacteria in Mashhad, we decided to investigate about TEM, CTX and SHV ESBLs among urinary Escherichia coli isolates in Mashhad, a city in northeast Iran. Materials and Methods ...

متن کامل

Determining frequency of Escherichia coli producing beta lactamase CTX-M and SHV in hospital and non-hospital clinical specimens in Isfahan

Background and aim: Antimicrobial resistance is a worldwide problem causing health threats. Escherichia coli is one of the most important bacteria that causes resistance problems. The aim of this study was to evaluate the frequency of Escherichia coli, a extended spectrum beta-lactamase producing CTX-M and SHV, in hospital and non-hospital clinical specimens in Isfahan.   Methods: In this stu...

متن کامل

Antibiotic Susceptibility and Multi-drug Resistance of Escherichia coli Isolates Producing CTX-M and TEM Type Beta-lactamases in Mashhad, Iran, in 2010

Background & Aims: One of the most common causes of bacterial resistance to beta-lactam antibiotics is beta-lactamase producing. The aim of this study was to compare the antibiotic resistance of urinary Escherichia coli (E. coli) isolates producing CTX-M and TEM type beta-lactamases, and to determine the strains with co-resistance to multiple antibiotics in Mashhad, Iran. Methods: E. coli b...

متن کامل

Molecular epidemiology of CTX-M-producing Escherichia coli isolates at a tertiary medical center in western Pennsylvania.

A combination of phenotypic and genotypic methods was used to investigate 70 unique Escherichia coli clinical isolates identified as producing extended-spectrum beta-lactamases (ESBLs) at a medical center in Pittsburgh, PA, between 2007 and 2008. Fifty-seven isolates (81%) produced CTX-M-type ESBLs, among which CTX-M-15 was predominant (n = 46). Isolates producing CTX-M-2, -9, -14, and -65 were...

متن کامل

Antibiotic Resistance Pattern and Genotype of Beta-Lactamase Producing Escherichia coli Isolates from Urinary Tract Infections in Zabol-Souteast of Iran

Introduction: Extended spectrum beta-lactamase (ESBL) producing Escherichia coli generate a major problem for clinical therapeutics and epidemiological study. The incidence of ESBL producing strains among clinical isolates has been steadily increasing during the past few years, and remains an important cause of failure of therapy with cephalosporins. The aim of this study was to determine the a...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

دوره 48 6  شماره 

صفحات  -

تاریخ انتشار 2009